6JFL
Nucleotide-free Mitofusin2 (MFN2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.482, 128.424, 91.329 |
Unit cell angles | 90.00, 106.27, 90.00 |
Refinement procedure
Resolution | 36.337 - 2.806 |
R-factor | 0.2108 |
Rwork | 0.207 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jfm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.184 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.700 | 2.980 |
High resolution limit [Å] | 2.800 | 2.810 |
Rmeas | 0.128 | 0.586 |
Number of reflections | 44958 | 6966 |
<I/σ(I)> | 10.42 | |
Completeness [%] | 98.0 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.19 M Calcium chloride 0.095 M HEPES PH7.5 26.6% (V/V) PEG400 5% (V/V) Glycerol |