6JFK
GDP bound Mitofusin2 (MFN2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.977760 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 49.624, 49.624, 388.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.223 - 1.997 |
R-factor | 0.1782 |
Rwork | 0.176 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gom |
RMSD bond length | 0.007 |
RMSD bond angle | 0.903 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.600 | 2.120 |
High resolution limit [Å] | 1.997 | 2.000 |
Rmeas | 0.064 | |
Rpim | 0.520 | |
Number of reflections | 34454 | 5321 |
<I/σ(I)> | 18.31 | |
Completeness [%] | 99.3 | |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Lithium citrate tribasic tetrahydrate, 35% (V/V) Glycerol ethoxylate |