6JFB
Crystal structure of human pyruvate kinase M2 isoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-16 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.763, 152.573, 94.405 |
| Unit cell angles | 90.00, 97.88, 90.00 |
Refinement procedure
| Resolution | 29.960 - 2.120 |
| R-factor | 0.19489 |
| Rwork | 0.192 |
| R-free | 0.25071 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 121473 | |
| <I/σ(I)> | 26.12 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Tris, pH 7.5, 0.2 M NaCl, 14% w/v polyethylene glycol 3350 |
