6JF1

Crystal structure of the substrate binding protein of a methionine transporter from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-02-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	54.692, 75.851, 75.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.910 - 1.950
R-factor	0.18569
Rwork	0.183
R-free	0.23062
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p99
RMSD bond length	0.012
RMSD bond angle	1.564
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.129	0.800
Number of reflections	22518
<I/σ(I)>	10.42	2.02
Completeness [%]	96.0	71.7
Redundancy	3.6	2.3
CC(1/2)	0.992	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 8000, MES sodium salt pH 6.5, zinc acetate