6JE7
Crystal structure of human serum albumin crystallized by ammonium sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.969, 59.024, 84.857 |
| Unit cell angles | 90.00, 113.42, 90.00 |
Refinement procedure
| Resolution | 29.064 - 3.900 |
| R-factor | 0.2915 |
| Rwork | 0.290 |
| R-free | 0.32570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ao6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 32372 | |
| <I/σ(I)> | 14.046 | |
| Completeness [%] | 82.9 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 1.2M ammonium sulfate, 1%(v/v) 1,4-1,1,1,3,3,3-Hexafluoro-2-propanol |
