6JDS
Crystal structure of truncated PRRSV nsp10 (helicase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-09-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 99.976, 99.976, 83.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21215 |
| Rwork | 0.211 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jdr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.846 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | |
| Rmeas | 0.083 | 0.858 |
| Rpim | 0.022 | 0.226 |
| Number of reflections | 15236 | 703 |
| <I/σ(I)> | 36.44 | |
| Completeness [%] | 99.6 | 93.6 |
| Redundancy | 14.5 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 5000 MME, 1-propanol, MES |
