6JDP
the imm52 family protein TsiT (PA3908) from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | H 3 |
| Unit cell lengths | 86.960, 86.960, 81.108 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.740 - 1.601 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.22010 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.052 | 0.752 |
| Number of reflections | 30138 | 1610 |
| <I/σ(I)> | 19.417 | 1.222 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 | |
| CC(1/2) | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.26 M Sodium phosphate monobasic monohydrate, 0.14 M Potassium phosphate dibasic |
