6JDL
Central domain of FleQ H287A mutant in complex with ATPgS and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 65 |
| Unit cell lengths | 105.011, 105.011, 43.191 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.471 - 2.249 |
| R-factor | 0.1775 |
| Rwork | 0.174 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5exp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.169 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.320 |
| High resolution limit [Å] | 2.249 | 2.250 |
| Rmerge | 0.096 | 0.800 |
| Number of reflections | 13175 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 0.2M SODIUM THIOCYANATE, 22% PEG 3350 |
