6JDJ

Crystal structure of AcrIIC2 dimer in complex with partial Nme1Cas9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97891
Spacegroup name	P 21 21 21
Unit cell lengths	61.439, 77.347, 106.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.673 - 2.600
R-factor	0.1954
Rwork	0.194
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jd7
RMSD bond length	0.005
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.154	0.900
Rmeas	0.161	0.952
Rpim	0.045	0.301
Number of reflections	16424	803
<I/σ(I)>	2.3	2.3
Completeness [%]	99.9	99.4
Redundancy	12.1	9.4
CC(1/2)	0.995	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	289	0.1M HEPES 7.9, 14% PEG 20000