6JD7

Crystal structure of anti-CRISPR protein AcrIIC2 dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	105.559, 73.532, 81.087
Unit cell angles	90.00, 129.80, 90.00

Refinement procedure

Resolution	39.992 - 2.450
R-factor	0.1941
Rwork	0.194
R-free	0.19700
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.479
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.074	0.288
Rmeas	0.083	0.321
Rpim	0.037
Number of reflections	17826	855
<I/σ(I)>	19.7	4.57
Completeness [%]	99.7	99.7
Redundancy	5.1	5.1
CC(1/2)	0.988	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	6.5	289	0.05M Na cacodylate pH 6.5, 0.1M Ammonium acetate, 0.015M Mg acetate, 10% Isopropanol