6JCO

Crystal structure of calcium free human gelsolin amyloid mutant D187N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 4 21 2
Unit cell lengths	170.130, 170.130, 151.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.193 - 2.880
R-factor	0.2088
Rwork	0.206
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ffn
RMSD bond length	0.010
RMSD bond angle	1.109
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.193	2.980
High resolution limit [Å]	2.880	2.880
Number of reflections	50758
<I/σ(I)>	3.51
Completeness [%]	99.9
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298.1	15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5