6JC4
Crystal structure of the urease accessory protein UreF from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 4 |
Unit cell lengths | 121.886, 121.886, 63.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.030 - 2.300 |
R-factor | 0.20166 |
Rwork | 0.200 |
R-free | 0.24172 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.547 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0241) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.034 | 0.142 |
Number of reflections | 41752 | 2055 |
<I/σ(I)> | 27.6 | 9.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.5 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% (w/v) PEG-8000, 0.1M CHES, pH 9.5, 0.2M NaCl |