6JBW
Structure of Tps1/UDP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 92.751, 99.295, 121.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.650 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uqu |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.970 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.077 | |
Rpim | 0.023 | |
Number of reflections | 33303 | 3263 |
<I/σ(I)> | 36.55 | |
Completeness [%] | 99.7 | |
Redundancy | 6.6 | |
CC(1/2) | 1.000 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, 20% PEG 3350 (v/v) |