6JBS

Bifunctional xylosidase/glucosidase LXYL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97925
Spacegroup name	P 43 21 2
Unit cell lengths	131.913, 131.913, 385.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.188
Rwork	0.184
R-free	0.25100
RMSD bond length	0.009
RMSD bond angle	1.527
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.155
Number of reflections	126287
<I/σ(I)>	23.6
Completeness [%]	99.7	98.61
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	15% PEG 3350, 0.1M Hepes pH 7.5, 0.2% MgCl2