6JBR
Tps1/UDP/T6P complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.279, 172.493, 141.703 |
Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
Resolution | 29.798 - 2.030 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uqt |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.057 | 0.451 |
Rpim | 0.023 | 0.195 |
Number of reflections | 305744 | 30304 |
<I/σ(I)> | 32.26 | |
Completeness [%] | 99.6 | |
Redundancy | 3.5 | |
CC(1/2) | 1.000 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 289 | 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, 20% PEG 3350 (v/v) |