6JBK
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-02-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.220, 81.192, 89.869 |
| Unit cell angles | 90.62, 107.19, 109.63 |
Refinement procedure
| Resolution | 19.965 - 2.450 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ypu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.002 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.210 | 2.493 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 49603 | |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 94.5 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298.15 | 20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350 |
