6JBI

Structure of Tps1 apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-26
Detector	MARRESEARCH
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	99.130, 121.480, 93.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.716 - 2.500
R-factor	0.2291
Rwork	0.226
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uqt
RMSD bond length	0.007
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.071
Rpim	0.020
Number of reflections	38668	3843
<I/σ(I)>	23.6
Completeness [%]	97.7
Redundancy	13.7
CC(1/2)	1.000	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	289	0.2 M Ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, 20% PEG 3350