6JBB
Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287A in complex with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-04-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.480, 84.480, 145.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.110 - 1.950 |
R-factor | 0.1444 |
Rwork | 0.143 |
R-free | 0.17690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j9w |
RMSD bond length | 0.017 |
RMSD bond angle | 1.796 |
Data reduction software | iMOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.110 | 8.940 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.118 | 0.557 |
Rmeas | 0.133 | 0.629 |
Rpim | 0.061 | 0.285 |
Number of reflections | 38891 | 2614 |
<I/σ(I)> | 13.4 | 3.2 |
Completeness [%] | 99.0 | 96.9 |
Redundancy | 8 | 7.9 |
CC(1/2) | 0.997 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.4 | 277 | 0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350 |