6JBA

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287A in complex with palatinose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.100, 84.100, 146.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.880 - 2.000
R-factor	0.1525
Rwork	0.151
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.017
RMSD bond angle	1.787
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.880	8.940
High resolution limit [Å]	2.000	2.000
Rmerge	0.140	0.574
Rmeas	0.158	0.653
Rpim	0.073	0.308
Number of reflections	36237	2647
<I/σ(I)>	10.4	3.4
Completeness [%]	100.0	100
Redundancy	8.5	8.4
CC(1/2)	0.990	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350