6JB9
Crystal structure of nanobody D3-L11 (unbound form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.190, 53.360, 36.210 |
Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
Resolution | 26.690 - 1.150 |
R-factor | 0.10211 |
Rwork | 0.102 |
R-free | 0.12903 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jb2 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.167 |
Data reduction software | MOSFLM (7.1.0) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.700 | 1.210 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.070 | 0.210 |
Number of reflections | 34665 | 4414 |
<I/σ(I)> | 14.1 | 6 |
Completeness [%] | 97.9 | 85.9 |
Redundancy | 5.2 | |
CC(1/2) | 0.997 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 100mM TRIS HCl, 100mM NaCl, 2.0M Ammonium sulfate |