6JAZ
Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287F in complex with trehalose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.270, 85.270, 145.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.590 - 1.850 |
| R-factor | 0.1344 |
| Rwork | 0.133 |
| R-free | 0.16830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j9w |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.835 |
| Data reduction software | iMOSFLM (7.2.2) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.590 | 9.060 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.092 | 0.511 |
| Rmeas | 0.098 | 0.547 |
| Rpim | 0.035 | 0.196 |
| Number of reflections | 46653 | 2839 |
| <I/σ(I)> | 21.6 | 5.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.8 | 14.5 |
| CC(1/2) | 0.999 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.4 | 277 | 0.04 M Citric Acid, 0.06 M Bis-Tris Propne, 20% PEG 3350 |
