6JAQ

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.620, 84.620, 146.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.240 - 1.950
R-factor	0.1332
Rwork	0.131
R-free	0.16720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.019
RMSD bond angle	1.783
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.240	8.940
High resolution limit [Å]	1.950	1.950
Rmerge	0.108	0.483
Rmeas	0.116	0.517
Rpim	0.041	0.185
Number of reflections	39565	2725
<I/σ(I)>	22.2	6
Completeness [%]	100.0	100
Redundancy	14.7	14.7
CC(1/2)	0.999	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350