6JAI

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein D118A in complex with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-02-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.720, 84.720, 146.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.280 - 2.100
R-factor	0.1441
Rwork	0.142
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.017
RMSD bond angle	1.705
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.280	8.910
High resolution limit [Å]	2.100	2.100
Rmerge	0.126	0.481
Rmeas	0.134	0.514
Rpim	0.046	0.180
Number of reflections	31860	2553
<I/σ(I)>	17.5	6
Completeness [%]	100.0	100
Redundancy	15.5	15.1
CC(1/2)	0.998	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350