6JAH

Crystal structure of ABC transporter alpha-glycoside-binding protein in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 41 21 2
Unit cell lengths	84.230, 84.230, 145.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.950 - 1.630
R-factor	0.136
Rwork	0.135
R-free	0.16050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.021
RMSD bond angle	1.990
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.950	8.920
High resolution limit [Å]	1.630	1.630
Rmerge	0.082	0.647
Rmeas	0.087	0.690
Rpim	0.028	0.239
Number of reflections	66385	3266
<I/σ(I)>	22.3	4.3
Completeness [%]	100.0	100
Redundancy	17.8	15.9
CC(1/2)	0.999	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5	277	0.05 M Citric Acid, 0.05 Bis-Tris Propane, 16% PEG 3350