6JAG

Crystal structure of ABC transporter alpha-glycoside-binding protein in complex with sucrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.930, 84.930, 145.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.410 - 1.850
R-factor	0.1312
Rwork	0.129
R-free	0.16680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.019
RMSD bond angle	1.801
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.410	9.060
High resolution limit [Å]	1.850	1.850
Rmerge	0.107	0.573
Rmeas	0.113	0.608
Rpim	0.036	0.203
Number of reflections	46411	2826
<I/σ(I)>	24.3	5.8
Completeness [%]	100.0	100
Redundancy	17.7	16.7
CC(1/2)	0.999	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350