6JAE
Crystal structure of Trypanosoma brucei gambiense glycerol kinase complex with Pi (pyrophosphatase reaction)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.309, 122.229, 155.958 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.300 |
| R-factor | 0.21139 |
| Rwork | 0.209 |
| R-free | 0.26462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wxl |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.706 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.020 | 0.223 |
| Number of reflections | 103029 | 31789 |
| <I/σ(I)> | 19.4 | 2.8 |
| Completeness [%] | 95.8 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 8-18% PEG 400, 0.1M HEPES, 11% 1,6-HEXANEDIOL |
