6J9W

Crystal structure of ABC transporter alpha-glycoside-binding protein in complex with trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-06-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.900, 84.900, 145.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.370 - 1.800
R-factor	0.1341
Rwork	0.133
R-free	0.16410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eu8
RMSD bond length	0.020
RMSD bond angle	1.848
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.370	9.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.088	0.435
Rmeas	0.095	0.473
Rpim	0.037	0.185
Number of reflections	50048	2860
<I/σ(I)>	18.8	5.8
Completeness [%]	99.8	98.9
Redundancy	12.2	12.3
CC(1/2)	0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 20% PEG 3350