6J9V
Crystal structure of Trypanosoma brucei gambiense glycerol kinase complex with ADP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.416, 120.948, 154.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.19653 |
Rwork | 0.193 |
R-free | 0.25416 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wxl |
RMSD bond length | 0.014 |
RMSD bond angle | 1.763 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.054 | 0.654 |
Number of reflections | 46371 | 14037 |
<I/σ(I)> | 15.71 | 2.1 |
Completeness [%] | 99.8 | 99 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 8-18% PEG 400, 0.1M HEPES, 11% 1,6-HEXANEDIOL |