6J9H

Crystal structure of SVBP-VASH1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.516, 93.372, 126.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.201 - 2.310
R-factor	0.2488
Rwork	0.248
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j91
RMSD bond length	0.007
RMSD bond angle	1.376
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.14_3260: 000))
Refinement software	PHENIX ((1.14_3260: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.030	2.430
High resolution limit [Å]	2.310	2.310
Rpim	0.075	0.428
Number of reflections	37912	5413
<I/σ(I)>	8.8	3.4
Completeness [%]	99.9	99.9
Redundancy	12.6	13.3
CC(1/2)		0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Na citrate tribasic dihydrate pH 5.5, 16% PEG 8000, 0.01M Cadmium chloride hydrate