6J9D

Babesia microti lactate dehydrogenase R99A (BmLDHR99A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 160 kV
Temperature [K]	277
Detector technology	CCD
Collection date	2018-12-15
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97890
Spacegroup name	P 32 2 1
Unit cell lengths	147.332, 147.332, 65.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.715 - 2.904
R-factor	0.2909
Rwork	0.289
R-free	0.32170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.692
Data reduction software	HKL-2000 (V718)
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.071	0.071
Rmeas	0.081	0.081
Rpim	0.038	0.038
Number of reflections	18199	18429
<I/σ(I)>	2.69	2.69
Completeness [%]	98.9
Redundancy	13
CC(1/2)	0.989	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Calcium Chloride, 0.1 M Tris:HCl pH 8.5, 15-18% (w/v) PEG 4000.