6J9A

Crystal structure of Arabidopsis thaliana VAL1 in complex with FLC DNA fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 43
Unit cell lengths	59.157, 59.157, 97.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.731 - 2.915
R-factor	0.2063
Rwork	0.204
R-free	0.24260
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.145
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.119	1.072
Number of reflections	5228
<I/σ(I)>	27.9	2.1
Completeness [%]	100.0	100
Redundancy	13.6	14
CC(1/2)		0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M LiCl, 20% PEG 3350