6J98
Crystal structure of P8 from Lactobacillus rhamnosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-17 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9738 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.860, 40.860, 107.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.170 - 1.560 |
R-factor | 0.17862 |
Rwork | 0.178 |
R-free | 0.19622 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.781 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.170 | 1.601 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.111 | 0.600 |
Rpim | 0.180 | |
Number of reflections | 12894 | 925 |
<I/σ(I)> | 4.95 | |
Completeness [%] | 99.7 | 98.98 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 290 | 0.05M Zinc Acetate dihydrate, 20% PEG3350, 5% n-dodecyl-N, N-dimethylamine-N-oxide |