6J8W
Structure of MOEN5-SSO7D fusion protein in complex with lig 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 137.777, 217.673, 104.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.350 |
| R-factor | 0.17777 |
| Rwork | 0.175 |
| R-free | 0.22488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b02 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.420 |
| High resolution limit [Å] | 2.340 | 5.030 | 2.340 |
| Rmerge | 0.096 | 0.045 | 0.827 |
| Rmeas | 0.103 | 0.048 | 0.894 |
| Rpim | 0.039 | 0.019 | 0.338 |
| Total number of observations | 461075 | ||
| Number of reflections | 65630 | 6826 | 6535 |
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7 | 6.8 | 6.9 |
| CC(1/2) | 0.998 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 298 | 0.2M LI3-CITRATE, 0.3M NACL, 25% W/V PEG 3350 |
