6J87
Crystal structure of HinD with NMFT and NO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-30 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.212, 155.029, 56.556 |
Unit cell angles | 90.00, 115.54, 90.00 |
Refinement procedure
Resolution | 48.472 - 2.300 |
R-factor | 0.1965 |
Rwork | 0.178 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j82 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.116 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.472 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.106 | 0.549 |
Number of reflections | 33169 | 3239 |
<I/σ(I)> | 7.1 | 1.8 |
Completeness [%] | 98.0 | 98 |
Redundancy | 2.9 | 3 |
CC(1/2) | 0.987 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES (pH 6.5) containing 1200 mM (NH4)2HPO4 and 100 mM NaCl |