6J85
Crystal structure of HinD apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-30 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.356, 152.647, 56.394 |
Unit cell angles | 90.00, 115.85, 90.00 |
Refinement procedure
Resolution | 48.157 - 2.200 |
R-factor | 0.1819 |
Rwork | 0.170 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j82 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.982 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.160 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.118 | 0.629 |
Number of reflections | 37990 | 3318 |
<I/σ(I)> | 8.5 | 2.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.9 | 3.9 |
CC(1/2) | 0.993 | 0.652 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES (pH 6.5) containing 1200 mM (NH4)2HPO4 and 100 mM NaCl |