6J83
Crystal structure of TleB with NMVT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.787, 84.761, 113.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.034 - 1.900 |
R-factor | 0.1757 |
Rwork | 0.173 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j82 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.904 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.034 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.168 | 0.446 |
Number of reflections | 31014 | 1941 |
<I/σ(I)> | 8.3 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.3 |
CC(1/2) | 0.983 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES (pH 6.0) containing 1600 mM Na/Ka tartrate and 5% 1, 4-dioxane |