6J7Z
Human mitochondrial Oligoribonuclease in complex with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-11 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.192, 125.892, 86.008 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.405 - 2.005 |
R-factor | 0.2072 |
Rwork | 0.206 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2igi |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.076 | 0.046 | 0.508 |
Rmeas | 0.083 | 0.050 | 0.549 |
Rpim | 0.033 | 0.020 | 0.208 |
Number of reflections | 31548 | 1604 | 1540 |
<I/σ(I)> | 17.1 | ||
Completeness [%] | 99.6 | 94.2 | 100 |
Redundancy | 6.5 | 5.6 | 6.9 |
CC(1/2) | 0.997 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.2 M Ammonium citrate dibasic, 20% w/v PEG 3350 |