6J7S
Crystal structure of toxin TglT (unusual type guanylyltransferase-like toxin, Rv1045) wild type protein from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.890, 95.890, 68.886 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.560 - 2.102 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j7t |
RMSD bond length | 0.004 |
RMSD bond angle | 0.731 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.560 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.081 | 0.746 |
Number of reflections | 21592 | 3430 |
<I/σ(I)> | 23.47 | 3.18 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 11.04 | 11.23 |
CC(1/2) | 0.999 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 0.1 M Bis-Tris pH 6.5, 0.2 M magnesium chloride hexahydrate, 25% polyethylene glycol 3350 (v/v). |