6J7N
Crystal structure of toxin TglT (unusual type guanylyltransferase-like toxin, Rv1045) mutant D82A co-expressed with TakA from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-01 |
Detector | DECTRIS EIGER X 1M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.502, 95.502, 68.183 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.750 - 2.294 |
R-factor | 0.2141 |
Rwork | 0.211 |
R-free | 0.26720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j7t |
RMSD bond length | 0.007 |
RMSD bond angle | 0.832 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.750 | 2.430 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.078 | 0.906 |
Number of reflections | 29779 | 4746 |
<I/σ(I)> | 7.25 | 0.87 |
Completeness [%] | 95.6 | 94.7 |
Redundancy | 2.8 | 2.74 |
CC(1/2) | 0.994 | 0.478 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M Bis-Tris pH=6.5, 0.2 M magnesium chloride hexahydrate and 25% polyethylene glycol 3,350 (v/v). |