6J7J

Crystal structure of Pseudomonas aeruginosa Earp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	45.217, 93.183, 119.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.523 - 1.750
R-factor	0.1769
Rwork	0.175
R-free	0.20910
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rmerge	0.083	0.050	0.993
Rmeas	0.090	0.055	1.070
Rpim	0.033	0.021	0.397
Total number of observations	369193
Number of reflections	51672	2757	2548
<I/σ(I)>	8.7
Completeness [%]	98.9	96.7	99.8
Redundancy	7.1	6.5	7
CC(1/2)		0.997	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH6.5, 12% PEG20K