6J7H
Crystal structure of blue fluorescent protein from metagenomic library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.281, 90.564, 171.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.347 - 2.309 |
R-factor | 0.2068 |
Rwork | 0.205 |
R-free | 0.25610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v2g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.870 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.088 | 0.327 |
Number of reflections | 39207 | 1928 |
<I/σ(I)> | 17.8 | |
Completeness [%] | 99.8 | |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 0.1M BICINE pH 9.0, 0.3M Magnesium nitrate, 22% w/v PEG 2000, 4% MPD |