6J7B
Crystal structure of VASH1-SVBP in complex with epoY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 44.310, 70.920, 128.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.540 - 1.618 |
R-factor | 0.1789 |
Rwork | 0.178 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j4o |
RMSD bond length | 0.007 |
RMSD bond angle | 0.904 |
Data reduction software | iMOSFLM |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.920 | 1.710 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 1.326 | |
Rpim | 0.039 | 0.390 |
Number of reflections | 52501 | 7575 |
<I/σ(I)> | 12.8 | |
Completeness [%] | 100.0 | |
Redundancy | 12 | |
CC(1/2) | 0.998 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 293 | 20%PEG6000, 1M lithium chloride, 0.1M MES pH6.0 |