6J76
Structure of 3,6-anhydro-L-galactose Dehydrogenase in Complex with NAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 76.378, 90.737, 149.052 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.984 - 2.368 |
R-factor | 0.1626 |
Rwork | 0.160 |
R-free | 0.20440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j75 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.053 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.453 |
High resolution limit [Å] | 2.368 | 2.368 |
Rmerge | 0.165 | 0.340 |
Number of reflections | 42618 | 3935 |
<I/σ(I)> | 44.27 | |
Completeness [%] | 99.0 | |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | NaAC, sodium cacodylate trihydrate, PEG 8,000 |