6J6U
Rat PTPRZ D1-D2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 127.725, 147.585, 65.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.970 - 3.320 |
| R-factor | 0.23925 |
| Rwork | 0.235 |
| R-free | 0.31787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nlk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.410 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Number of reflections | 15767 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 84.0 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 14% PEG3350, 0.15M potassium fluoride, bis-Tris propane pH6.5 |
