6J6U
Rat PTPRZ D1-D2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-10-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 127.725, 147.585, 65.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.970 - 3.320 |
R-factor | 0.23925 |
Rwork | 0.235 |
R-free | 0.31787 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nlk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.410 |
High resolution limit [Å] | 3.320 | 3.320 |
Number of reflections | 15767 | |
<I/σ(I)> | 13 | |
Completeness [%] | 84.0 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 14% PEG3350, 0.15M potassium fluoride, bis-Tris propane pH6.5 |