6J6C
Borrelia burgdorferi OspA via surface entropy reduction (form3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.197, 54.316, 65.773 |
| Unit cell angles | 90.00, 100.49, 90.00 |
Refinement procedure
| Resolution | 19.715 - 1.600 |
| R-factor | 0.1879 |
| Rwork | 0.187 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.422 |
| Number of reflections | 30494 | 1490 |
| <I/σ(I)> | 23.4 | 1.92 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1 M Imidazole pH 7.0, 45% PEG 400 |
