6J6A

Crystal structure of TldE from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	104.497, 104.497, 253.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.931 - 2.353
R-factor	0.1966
Rwork	0.196
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vl4
RMSD bond length	0.008
RMSD bond angle	1.014
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc2_3191: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.931	2.437
High resolution limit [Å]	2.353	2.353
Rpim	0.052	0.508
Number of reflections	59216	2896
<I/σ(I)>	29.67
Completeness [%]	99.9
Redundancy	11.1
CC(1/2)	0.996	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.02M Calcium Chloride, 0.1M Sodium Acetate pH 4.6, 30% v/v MPD