6J69
Structure of KIBRA and Dendrin Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-01-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97774 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 85.443, 85.443, 80.566 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.721 - 2.753 |
R-factor | 0.2386 |
Rwork | 0.236 |
R-free | 0.28240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j68 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.201 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.753 | 2.753 |
Rmerge | 0.071 | 0.920 |
Number of reflections | 8167 | 391 |
<I/σ(I)> | 38.6 | |
Completeness [%] | 99.8 | |
Redundancy | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1M HEPES, 0.4M Sodium citrate, 16% 2-Propanol |