6J69
Structure of KIBRA and Dendrin Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97774 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 85.443, 85.443, 80.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.721 - 2.753 |
| R-factor | 0.2386 |
| Rwork | 0.236 |
| R-free | 0.28240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j68 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.753 | 2.753 |
| Rmerge | 0.071 | 0.920 |
| Number of reflections | 8167 | 391 |
| <I/σ(I)> | 38.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1M HEPES, 0.4M Sodium citrate, 16% 2-Propanol |
