6J62

Crystal structure of mISG15/NS1B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	108.113, 37.766, 72.531
Unit cell angles	90.00, 101.35, 90.00

Refinement procedure

Resolution	35.580 - 2.486
R-factor	0.2077
Rwork	0.204
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.580	2.570
High resolution limit [Å]	2.480	2.480
Number of reflections	10221
<I/σ(I)>	14.4
Completeness [%]	98.0
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M magnesium chloride, 0.1M Na-HEPES, pH 7.0, 13 %(w/v) PEG 4000.