6J5R
OspA mutant, PSAM-VLGDV1-form8, grafted short chameleon sequence from alpha-B crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.280, 53.145, 64.651 |
| Unit cell angles | 90.00, 100.47, 90.00 |
Refinement procedure
| Resolution | 19.685 - 1.850 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j47 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.063 | 0.330 |
| Number of reflections | 18781 | 863 |
| <I/σ(I)> | 20.7 | 2.46 |
| Completeness [%] | 98.8 | 96.2 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M Tri-HCl pH 8.5, 38% PEG 400 |
