6J5Q
OspA mutant, PSAM-VLGDV1-form7, grafted short chameleon sequence from alpha-B crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.186, 53.536, 65.697 |
Unit cell angles | 90.00, 100.24, 90.00 |
Refinement procedure
Resolution | 19.685 - 1.798 |
R-factor | 0.2114 |
Rwork | 0.209 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b2a |
RMSD bond length | 0.006 |
RMSD bond angle | 0.799 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.067 | 0.643 |
Number of reflections | 23470 | 1166 |
<I/σ(I)> | 25.4 | 2.2 |
Completeness [%] | 97.9 | 95.3 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 0.1 M Tri-HCl pH 9.0, 38% PEG 400 |